11666-1-AP

antibody from Invitrogen Antibodies

Targeting: WSB1 DKFZp564A122, DKFZp564B0482, SWIP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11666-1-AP

Provider

Invitrogen Antibodies

Product name

WSB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MASFPPRVN EKEIVRLRTI GELLAPAAPF DKKCGRENWT VAFAPDGSYF AWSQGHRTVK LVPWSQCLQN FLLHGTKNVT NSSSLRLPRQ NSDGGQKNKP REHIIDCGDI VWSLAFGSSV PEKQSRCVNI EWHRFRFGQD QLLLATGLNN GRIKIWDVYT GKLLLNLVDH TEVVRDLTFA PDGSLILVSA SRDKTLRVWD LKDDGNMMKV LRGHQNWVYS CAFSPDSSML CSVGASKAVF LWNMDKYTMI RKLEGHHHDV VACDFSPDGA LLATASYDTR V (1-280 aa encoded by BC021110)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 11666-1-AP (WSB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 11666-1-AP (WSB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11666-1-AP (WSB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WSB1 antibody (Product # 11666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using WSB1 antibody 11666-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 11666-1-AP (WSB1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 11666-1-AP (WSB1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 11666-1-AP (WSB1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 11666-1-AP (WSB1 antibody) at dilution of 1:50 (under 40x lens).