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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- 16453-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNAJB5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGKDYYKIL GIPSGANEDE IKKAYRKMAL KYHPDKNKEP NAEEKFKEIA EAYDVLSDPK KRGLYDQYGE EGLKTGGGTS GGSSGSFHYT FHGDPHATFA SFFGGSNPFD IFFASSRSTR PFSGFDPDDM DVDEDEDPFG AFGRFGFNGL SRGPRRAPEP LYPRRKVQDP PVVHELRVSL EEIYHGSTKR MKITRRRLNP DGRTVRTEDK ILHIVIKRGW KEGTKITFPK EGDATPDNIP ADIVFVLKDK PHAHFRRDGT NVLYSALISL KEALCGCTVN IPTIDGRVIP LPCNDVIKPG TVKRLRGEGL PFPKVPTQRG DLIVEFKVRF PDRLTPQTRQ ILKQQYPQHS LHSMCTQLDV HWTLATFSKM QKKATGFQEN VPVPDPF (1-386 aa encoded by BC012115)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.16 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DNAJB5 (IP:16453-1-AP, 4ug; Detection:16453-1-AP 1:500) with HepG2 cells lysate 3300 ug.
