PAB29396

antibody from Abnova Corporation

Targeting: UBE2L6 UBCH8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29396

Provider

Abnova Corporation

Product name

UBE2L6 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human UBE2L6.

Antigen sequence

NLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFP
PEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSE
NWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADL
LTQNPELFRKNAEEFTLR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with UBE2L6 polyclonal antibody (Cat # PAB29396) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with UBE2L6 polyclonal antibody (Cat # PAB29396) at 1-4 ug/mL concentration shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human urinary bladder with UBE2L6 polyclonal antibody (Cat # PAB29396) shows strong cytoplasmic and nuclear positivity in urothelial cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)