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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-04351 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-04351, RRID:AB_1522639
- Product name
- Mouse Monoclonal UbcH8/Ube2L6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Cell lysates of MCF7 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2L6 Transfected 293T cell lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Flow cytometry analysis of UBE2L6 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
