Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00065975-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00065975-M02, RRID:AB_548447
- Product name
- Mouse Monoclonal STK33 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. STK33 - serine/threonine kinase 33
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Synthetic lethal interaction between oncogenic KRAS dependency and STK33 suppression in human cancer cells.
Scholl C, Fröhling S, Dunn IF, Schinzel AC, Barbie DA, Kim SY, Silver SJ, Tamayo P, Wadlow RC, Ramaswamy S, Döhner K, Bullinger L, Sandy P, Boehm JS, Root DE, Jacks T, Hahn WC, Gilliland DG
Cell 2009 May 29;137(5):821-34
Cell 2009 May 29;137(5):821-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi ( Cat # H00065975-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody (M02) clone 6G1 (Cat # H00065975-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M02), clone 6G1.Lane 1: STK33 transfected lysate(57.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STK33 Antibody (6G1) [H00065975-M02] - STK33 monoclonal antibody (M02), clone 6G1 Analysis of STK33 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: STK33 Antibody (6G1) [H00065975-M02] - Detection limit for recombinant GST tagged STK33 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: STK33 Antibody (6G1) [H00065975-M02] - Analysis of monoclonal antibody to STK33 on HeLa cell. Antibody concentration 25 ug/ml.