12857-1-AP

antibody from Invitrogen Antibodies

Targeting: STK33

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12857-1-AP

Provider

Invitrogen Antibodies

Product name

STK33 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MADSGLDKK STKCPDCSSA SQKDVLCVCS SKTRVPPVLV VEMSQTSSIG SAESLISLER KKEKNINRDI TSRKDLPSRT SNVERKASQQ QWGRGNFTEG KVPHIRIENG AAIEEIYTFG RILGKGSFGI VIEATDKETE TKWAIKKVNK EKAGSSAVKL LEREVNILKS VKHEHIIHLE QVFETPKKMY LVMELCEDGE LKEILDRKGH FSENETRWII QSLASAIAYL HNNDIVHRDL KLENIMVKSS LIDDNNEINL NIKVTDFGLA VKKQSRSEAM LQATCGTPIY MAPEVISAHD YSQQCDIWSI GVVMYMLLRG EPPFLASSEE KLFELIRKGE LHFENAVWNS ISDCAKSVLK QLMKVDPAHR ITAKELLDNQ WL (1-381 aa encoded by BC031231)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12857-1-AP (STK33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12857-1-AP (STK33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human kidney tissue were subjected to SDS PAGE followed by western blot with 12857-1-AP (STK33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed A431 cells using 12857-1-AP (STK33 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12857-1-AP (STK33 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12857-1-AP (STK33 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12857-1-AP (STK33 antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-STK33 (IP:12857-1-AP, 4ug; Detection:12857-1-AP 1:300) with HEK-293 cells lysate 880ug.