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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Proximity ligation assay [1]
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- Product number
- H00005494-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005494-M03, RRID:AB_1112045
- Product name
- PPM1A monoclonal antibody (M03), clone 4E11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant PPM1A.
- Antigen sequence
MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVE
MEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKY
CCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGF
LEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTY
FINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERI
QNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGP
TEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVM
GNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGS
RDNMSVILICFPNAPKVSPEAVKKEAELDKYLECR
VEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGE
LASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW- Isotype
- IgG
- Antibody clone number
- 4E11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PPM1A is 3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PPM1A on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PPM1A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between AKT1 and PPM1A. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-PPM1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
