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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- NBP2-19935 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PRMT5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PRMT5 Antibody [NBP2-19935] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PRMT5 Antibody [NBP2-19935] - A. 35 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 ug PRMT5 antibody [N1C1] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [N1C1] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP): PRMT5 Antibody [NBP2-19935] - Cross-linked ChIP was performed with K562 chromatin extract and 5 ug of either control rabbit IgG or anti-PRMT5 antibody. The precipitated DNA was detected by PCR with primer set targeting to gamma-globin promoter.
