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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX116004 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116004, RRID:AB_10624553
- Product name
- PRMT5 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Protein arginine methyltransferase 5-mediated epigenetic silencing of IRX1 contributes to tumorigenicity and metastasis of gastric cancer.
Liu X, Zhang J, Liu L, Jiang Y, Ji J, Yan R, Zhu Z, Yu Y
Biochimica et biophysica acta. Molecular basis of disease 2018 Sep;1864(9 Pt B):2835-2844
Biochimica et biophysica acta. Molecular basis of disease 2018 Sep;1864(9 Pt B):2835-2844
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE GTX116004 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRMT5 antibody [N1C1] immunoprecipitates PRMT5 protein in IP experiments.IP samples: Raji whole cell extractA. 35 £gg Raji whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of PRMT5 protein by 4 £gg PRMT5 antibody [N1C1] (GTX116004)7.5 % SDS-PAGEThe immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [N1C1] (GTX116004) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5(GTX116004) antibody at 1:100 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with K562 chromatin extract and 5 £gg of either control rabbit IgG or anti-PRMT5 antibody. The precipitated DNA was detected by PCR with primer set targeting to £^-globin promoter.
