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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2175 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2175, RRID:AB_1579189
- Product name
- PRMT5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRMT5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references mSin3A/histone deacetylase 2- and PRMT5-containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.
Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family.
The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity.
Pal S, Yun R, Datta A, Lacomis L, Erdjument-Bromage H, Kumar J, Tempst P, Sif S
Molecular and cellular biology 2003 Nov;23(21):7475-87
Molecular and cellular biology 2003 Nov;23(21):7475-87
Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family.
Rho J, Choi S, Seong YR, Cho WK, Kim SH, Im DS
The Journal of biological chemistry 2001 Apr 6;276(14):11393-401
The Journal of biological chemistry 2001 Apr 6;276(14):11393-401
The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity.
Pollack BP, Kotenko SV, He W, Izotova LS, Barnoski BL, Pestka S
The Journal of biological chemistry 1999 Oct 29;274(44):31531-42
The Journal of biological chemistry 1999 Oct 29;274(44):31531-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PRMT5 polyclonal antibody (Cat # PAB2175) is used in Western blot to detect PRMT5 in HL-60 cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
