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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504684 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504684, RRID:AB_2622524
- Product name
- UBE2E3 mouse monoclonal antibody, clone OTI4B4 (formerly 4B4)
- Antibody type
- Monoclonal
- Description
- UBE2E3 mouse monoclonal antibody, clone OTI4B4 (formerly 4B4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- UBE2E3
- Isotype
- IgG
- Antibody clone number
- OTI4B4
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-UBE2E3 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different mouse tissues by using anti-UBE2E3 monoclonal antibody. (1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-UBE2E3 mouse monoclonal antibody (TA504684) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2E3(RC210328).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504684)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-UBE2E3 antibody(TA504684),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
