NBP2-03819

antibody from Novus Biologicals

Targeting: UBE2E3 UbcH9

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-03819

Provider

Novus Biologicals

Product name

Mouse Monoclonal UBE2E3 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - (Negative UBE2E3 protein level by using anti-UBE2E3 antibody in HEK293E cells. Dilution: 1:150-500

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human colon tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human Kidney tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human pancreas tissue using anti-UBE2E3 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2E3 antibody, and then analyzed by flow cytometry.