PA5-142888

antibody from Invitrogen Antibodies

Targeting: USP6 HRP1, Tre-2, TRE17, Tre2, TRESMCR

Antibody data

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Validation data

Product number: PA5-142888 - Provider product page
Provider: Invitrogen Antibodies
Product name: USP6 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Additional Information: This antibody is expected NOT to cross react to USP32 or to the TBC1D3 proteins. This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:4,000. Preliminary analysis showed no signal in Western Blot.
Reactivity: Human
Host: Goat
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

USP6 protein structure - PA5-142888 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of USP6 in A431 cells using USP6 Polyclonal Antibody (Product # PA5-142888). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of USP6 in A431 cells using USP6 Polyclonal Antibody (Product # PA5-142888). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of USP6 in U2OS cells using USP6 Polyclonal Antibody (Product # PA5-142888). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).