AF6166

antibody from Novus Biologicals

Targeting: EOMES TBR2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6166

Provider

Novus Biologicals

Product name

Sheep Polyclonal EOMES Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human EOMES in Western blots.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Reprogramming to pluripotency does not require transition through a primitive streak-like state.

Raab S, Klingenstein M, Möller A, Illing A, Tosic J, Breunig M, Kuales G, Linta L, Seufferlein T, Arnold SJ, Kleger A, Liebau S
Scientific reports 2017 Nov 29;7(1):16543

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human EOMES by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 µg/mL of Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for EOMES at approximately 85-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of EOMES in Differ-entiated BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.