Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF1040 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-17RB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-17 RB in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-17 R and recombinant human IL-17 R is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9JIP3
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A novel regulatory function of CDKN1A/p21 in TNFα-induced matrix metalloproteinase 9-dependent migration and invasion of triple-negative breast cancer cells.
Zaremba-Czogalla M, Hryniewicz-Jankowska A, Tabola R, Nienartowicz M, Stach K, Wierzbicki J, Cirocchi R, Ziolkowski P, Tabaczar S, Augoff K
Cellular signalling 2018 Jul;47:27-36
Cellular signalling 2018 Jul;47:27-36
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Mouse IL-17 RB by Western Blot. Western blot shows lysates of mouse liver tissue and mouse spleen tissue (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL-17 RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1040) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-17 RB at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.