24474-1-AP

antibody from Invitrogen Antibodies

Targeting: C1QBP gC1Q-R, gC1qR, HABP1, p32, SF2p32

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

24474-1-AP

Provider

Invitrogen Antibodies

Product name

C1QBP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLPLLRCVP RVLGSSVAGL RAAAPASPFR QLLQPAPRLC TRPFGLLSVR AGSERRPGLL RPRGPCACGC GCGSLHTDGD KAFVDFLSDE IKEERKIQKH KTLPKMSGGW ELELNGTEAK LVRKVAGEKI TVTFNINNSI PPTFDGEEEP SQGQKVEEQE PELTSTPNFV VEVIKNDDGK KALVLDCHYP EDEVGQEDEA ESDIFSIREV SFQSTGESEW KDTNYTLNTD SLDWALYDHL MDFLADRGVD NTFADELVEL STALEHQEYI TFLEDLKSFV KSQ (1-282 aa encoded by BC013731)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 24474-1-AP ( C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NIH/3T3 cells using 24474-1-AP (C1QBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer slide using 24474-1-AP (C1QBP Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer slide using 24474-1-AP (C1QBP Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis slide using 24474-1-AP ( C1QBP Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis slide using 24474-1-AP (C1QBP Antibody) at dilution of 1:50.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human C1QBP (Product # 24474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.