- MA5-41061 - Invitrogen Antibodies C1QBP antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot of C1QBP in Lane A: C1QBP konckout Hela Whole Cell Lysate, Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 μg per lane. Samples (10 µg per lane) were incubated with monoclonal antibody (Product # MA5-41061) with a dilution of 1:500 , followed by Rabbit Anti-Mouse IgG F(ab)2/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 31 kDa, Observed band size: 35 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using C1QBP Monoclonal Antibody (04) (Product # MA5-41061) at 1:500 dilution. Lane A: C1QBP konckout Hela Whole Cell Lysate, Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 μg per lane. Secondary antibody: Rabbit Anti-Mouse IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot of C1QBP in Lane A: Hela Whole Cell lysate, Lane B: 293T Whole Cell lysate, Lane C: Jurkat Whole Cell lysate, Lane D: NIH/3T3 Whole Cell Lysate. Samples (30 µg per lane) were incubated with monoclonal antibody (Product # MA5-41061) with a dilution of 1:500 , followed by Goat Anti-Mouse IgG H&L (Dylight 800) using a dilution of 1:15,000. Assay was performed under reducing conditions. Predicted band size: 31 kDa, Observed band size: 35 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using C1QBP Monoclonal Antibody (04) (Product # MA5-41061) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: NIH/3T3 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 35 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence staining of Human C1QBP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS, blocked with 10% serum, and incubated with C1QBP Monoclonal Antibody (04) (Product # MA5-41061, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of C1QBP in Lane A: 0.5 mg Hela Whole Cell lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41061) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to Mouse IgG (H+L) using a dilution of 1:7,500. Assay was performed under reducing conditions. Predicted band size: 35 kDa, Observed band size: 35 kDa .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: C1QBP Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate 2 µL with C1QBP Monoclonal Antibody (04) (Product # MA5-41061) and 15 µL of 50 % Protein G agarose. Primary antibody: C1QBP Monoclonal Antibody (04), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7,500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa.

MA5-41061