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Western blot
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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- NB100-1081 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1081, RRID:AB_2127799
- Product name
- Goat Polyclonal Importin-7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome.
Nucleocytoplasmic shuttling of the zinc finger protein EZI Is mediated by importin-7-dependent nuclear import and CRM1-independent export mechanisms.
A novel class of RanGTP binding proteins.
A novel class of RanGTP binding proteins.
Braun DA, Sadowski CE, Kohl S, Lovric S, Astrinidis SA, Pabst WL, Gee HY, Ashraf S, Lawson JA, Shril S, Airik M, Tan W, Schapiro D, Rao J, Choi WI, Hermle T, Kemper MJ, Pohl M, Ozaltin F, Konrad M, Bogdanovic R, Büscher R, Helmchen U, Serdaroglu E, Lifton RP, Antonin W, Hildebrandt F
Nature genetics 2016 Apr;48(4):457-65
Nature genetics 2016 Apr;48(4):457-65
Nucleocytoplasmic shuttling of the zinc finger protein EZI Is mediated by importin-7-dependent nuclear import and CRM1-independent export mechanisms.
Saijou E, Itoh T, Kim KW, Iemura S, Natsume T, Miyajima A
The Journal of biological chemistry 2007 Nov 2;282(44):32327-37
The Journal of biological chemistry 2007 Nov 2;282(44):32327-37
A novel class of RanGTP binding proteins.
Görlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, Prehn S, Izaurralde E
The Journal of cell biology 1997 Jul 14;138(1):65-80
The Journal of cell biology 1997 Jul 14;138(1):65-80
A novel class of RanGTP binding proteins.
Görlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, Prehn S, Izaurralde E
The Journal of cell biology 1997 Jul 14;138(1):65-80
The Journal of cell biology 1997 Jul 14;138(1):65-80
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Enhanced validation
- Submitted by
- Novus Biologicals (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Knockdown Validated: Importin-7 Antibody [NB100-1081] - Western blot of C4-2 human prostate cancer cells 72hrs post transfection with 4 siRNAs targetting Importin 7 mRNA, along with non-target siRNA and reagent-only controls.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Importin-7 Antibody [NB100-1081] - Staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Importin-7 Antibody [NB100-1081] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
