13075-1-AP

antibody from Invitrogen Antibodies

Targeting: GSDME DFNA5, ICERE-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

13075-1-AP

Provider

Invitrogen Antibodies

Product name

DFNA5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SQSSFGTLR KQEVDLQQLI RDSAERTINL RNPVLQQVLE GRNEVLCVLT QKITTMQKCV ISEHMQVEEK CGGIVGIQTK TVQVSATEDG NVTKDSNVVL EIPAATTIAY GVIELYVKLD GQFEFCLLRG KQGGFENKKR IDSVYLDPLV FREFAFIDMP DAAHGISSQD GPLSVLKQAT LLLERNFHPF AELPEPQQTA LSDIFQAVLF DDELLMVLEP VCDDLVSGLS PTVAVLGELK PRQQQDLVAF LQLVGCSLQG GCPGPEDAGS KQLFMTAYFL VSALAEMPDS AAALLGTCCK LQIIPTLCHL LRALSDDGVS DLEDPTLTPL KDTERFGIVQ RLFASADISL (111-460 aa encoded by BC019689)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 13075-1-AP ( DFNA5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 13075-1-AP ( DFNA5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 µg Anti-Human DFNA5/GSDME (Product # 13075-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).