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- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- AF532 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TGF-beta RII Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) TGF-beta RII is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q62312- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.
miR-17∼92 family clusters control iNKT cell ontogenesis via modulation of TGF-β signaling.
Dendritic cell SIRT1-HIF1α axis programs the differentiation of CD4+ T cells through IL-12 and TGF-β1.
Intrinsic TGF-β signaling promotes age-dependent CD8+ T cell polyfunctionality attrition.
Augmented cytoplasmic Smad4 induces acceleration of TGF-beta1 signaling in renal tubulointerstitial cells of hereditary nephrotic ICGN mice with chronic renal fibrosis; possible role for myofibroblastic differentiation.
Lam J, van den Bosch M, Wegrzyn J, Parker J, Ibrahim R, Slowski K, Chang L, Martinez-Høyer S, Condorelli G, Boldin M, Deng Y, Umlandt P, Fuller M, Karsan A
Nature communications 2018 Jun 20;9(1):2418
Nature communications 2018 Jun 20;9(1):2418
miR-17∼92 family clusters control iNKT cell ontogenesis via modulation of TGF-β signaling.
Fedeli M, Riba M, Garcia Manteiga JM, Tian L, Viganò V, Rossetti G, Pagani M, Xiao C, Liston A, Stupka E, Cittaro D, Abrignani S, Provero P, Dellabona P, Casorati G
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 20;113(51):E8286-E8295
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 20;113(51):E8286-E8295
Dendritic cell SIRT1-HIF1α axis programs the differentiation of CD4+ T cells through IL-12 and TGF-β1.
Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 3;112(9):E957-65
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 3;112(9):E957-65
Intrinsic TGF-β signaling promotes age-dependent CD8+ T cell polyfunctionality attrition.
Bhadra R, Moretto MM, Castillo JC, Petrovas C, Ferrando-Martinez S, Shokal U, Leal M, Koup RA, Eleftherianos I, Khan IA
The Journal of clinical investigation 2014 Jun;124(6):2441-55
The Journal of clinical investigation 2014 Jun;124(6):2441-55
Augmented cytoplasmic Smad4 induces acceleration of TGF-beta1 signaling in renal tubulointerstitial cells of hereditary nephrotic ICGN mice with chronic renal fibrosis; possible role for myofibroblastic differentiation.
Goto Y, Manabe N, Uchio-Yamada K, Yamaguchi-Yamada M, Inoue N, Yamamoto Y, Ogura A, Nagano N, Miyamoto H
Cell and tissue research 2004 Feb;315(2):209-21
Cell and tissue research 2004 Feb;315(2):209-21
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TGF-beta RII by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF532) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TGF-beta RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
