Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-88257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGFBR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.27 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A novel circ_MACF1/miR-942-5p/TGFBR2 axis regulates the functional behaviors and drug sensitivity in gefitinib-resistant non-small cell lung cancer cells.
Fan D, Yang Y, Zhang W
BMC pulmonary medicine 2022 Jan 7;22(1):27
BMC pulmonary medicine 2022 Jan 7;22(1):27
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using TGFBR2 Polyclonal antibody (Product # PA5-88257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TGFBR2 in extracts of various cell lines using TGFBR2 Polyclonal Antibody (Product # PA5-88257) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of U2OS cells using TGFBR2 Polyclonal antibody (Product # PA5-88257) at dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TGFBR2 in paraffin-embedded human liver cancer using TGFBR2 Polyclonal Antibody (Product # PA5-88257) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TGFBR2 in paraffin-embedded human breast using TGFBR2 Polyclonal Antibody (Product # PA5-88257) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TGFBR2 in paraffin-embedded human mammary cancer using TGFBR2 Polyclonal Antibody (Product # PA5-88257) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 8 Overexpression of circ_MACF1 inhibits tumor growth under gefitinib in vivo. A qRT-PCR analysis of circ_MACF1 expression in A549/GR cells transduced by lentiviruses expressing circ_MACF1 (lenti-circ_MACF1) or a scrambled control (lenti-NC). A549/GR cells transduced by lenti-circ_MACF1 or lenti-NC were implanted into the right flanks of BALB/c nude mice (6 mice per group), with gefitinib administration (30 mg/kg) per os and tumor volume measurement ( B ) 10 days after implantation, and checked for tumor weight and images ( C ), circ_MACF1 expression by qRT-PCR analysis ( D ), miR-942-5p expression by qRT-PCR analysis ( E ), TGFBR2 mRNA level by qRT-PCR analysis ( F ), TGFBR2 protein level by western blot ( G ), TGFBR2 protein level and Ki67 staining by immunohistochemistry ( H ) at the end point (30 days after injection). * P < 0.05