PAB3272

antibody from Abnova Corporation

Targeting: EIF2AK1 HRI, KIAA1369

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3272

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3272, RRID:AB_1574600

Product name

EIF2AK1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of EIF2AK1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Autophosphorylation of threonine 485 in the activation loop is essential for attaining eIF2alpha kinase activity of HRI.

Rafie-Kolpin M, Han AP, Chen JJ
Biochemistry 2003 Jun 3;42(21):6536-44

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Regulation of protein synthesis by heme-regulated eIF-2 alpha kinase.

Chen JJ, London IM
Trends in biochemical sciences 1995 Mar;20(3):105-8

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of EIF2AK1 polyclonal antibody (Cat # PAB3272) in A-375 cell lysate. EIF2AK1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with EIF2AK1 polyclonal antibody (Cat # PAB3272) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)