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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-34624 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM23 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLaS3.
- Concentration
- 1 mg/mL
Submitted references Whole exome sequencing reveals the maintained polyclonal nature from primary to metastatic malignant peripheral nerve sheath tumor in two patients with NF1.
Godec A, Jayasinghe R, Chrisinger JSA, Prudner B, Ball T, Wang Y, Srihari D, Kaushal M, Dietz H, Zhang X, Pekmezci M, Dahiya S, Tao Y, Luo J, Van Tine BA, Ding L, Gutmann DH, Hirbe AC
Neuro-oncology advances 2020 Jul;2(Suppl 1):i75-i84
Neuro-oncology advances 2020 Jul;2(Suppl 1):i75-i84
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRIM23 was performed by separating 30 µg of HeLa S3 lysates by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a TRIM23 Polyclonal Antibody (Product # PA5-34624) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells using anti-TRIM23 polyclonal antibody (Product # PA5-34624) (Green) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TRIM23 using TRIM23 Polyclonal Antibody (Product # PA5-34624). Samples used were HCT116 cells mock (left) and cells treated with 30 µM Cisplatin for 24 hrs (right) fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 Polyclonal Antibody (Product # PA5-34624) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TRIM23 in paraformaldehyde-fixed HeLa cells using TRIM23 Polyclonal Antibody (Product # PA5-34624) (Green) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TRIM23 was performed in paraffin-embedded human gastric cancer tissue using TRIM23 Polyclonal Antibody (Product # PA5-34624) at a dilution of 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Identification of TRIM Family as potential drivers of progression and survival. (A) Kaplan-Meier survival curves for TRIM23 expression in sarcoma (SARC), glioblastoma (GBM), and breast carcinoma (BRCA) from TCGA GEPIA database. (B) Kaplan-Meier survival curves for TRIM23 expression from a cohort staining analysis of MPNST patients from Washington University in St. Louis and UCSF ( P = .15; n = 39). (C) Representative examples of TRIM23 staining in MPNST.
