MA5-44928

antibody from Invitrogen Antibodies

Targeting: KLRB1 CD161, CLEC5B, hNKR-P1A, NKR, NKR-P1, NKR-P1A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-44928

Provider

Invitrogen Antibodies

Product name

CD161 Monoclonal Antibody (A8E1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

A8E1

Vial size

100 µL

Concentration

1.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD161 in THP-1 cell lysates (10 µg/Lane). Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in CD161 Monoclonal antibody (Product # MA5-44928) using a dilution of 1:1,000 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:100,000 for 1 hour at room temperature. Predicted band size: 25 kDa. Observed band size: 25 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CD161 in paraffin-embedded human kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with CD161 Monoclonal antibody (Product # MA5-44928) using a dilution of 1:500 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CD161 in THP-1 cells. Cells were washed twice with cold PBS and resuspend then stained with CD161 Monoclonal antibody (Product # MA5-44928) using a dilution of 1 µg/mL (red) at 4℃ for 30 minutes followed by iFluor™ 488 conjugate-Goat anti-Mouse IgG secondary antibody at a dilution of 1:1,000 for 30 minutes at 4℃. Mouse IgG1 Isotype Control (green). Unlabeled sample was used as a control (cells without incubation with primary antibody; black).