MAB1934

antibody from Novus Biologicals

Targeting: PTPRJ CD148, DEP1, HPTPeta

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

MAB1934

Provider

Novus Biologicals

Product name

Mouse Monoclonal DEP-1/CD148 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human DEP-1/CD148 in Western blots.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

A novel splice variant of the protein tyrosine phosphatase PTPRJ that encodes for a soluble protein involved in angiogenesis.

Bilotta A, Dattilo V, D'Agostino S, Belviso S, Scalise S, Bilotta M, Gaudio E, Paduano F, Perrotti N, Florio T, Fusco A, Iuliano R, Trapasso F
Oncotarget 2017 Feb 7;8(6):10091-10102

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The protein tyrosine phosphatase DEP-1/PTPRJ promotes breast cancer cell invasion and metastasis.

Spring K, Fournier P, Lapointe L, Chabot C, Roussy J, Pommey S, Stagg J, Royal I
Oncogene 2015 Oct 29;34(44):5536-47

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Phosphorylation of DEP-1/PTPRJ on threonine 1318 regulates Src activation and endothelial cell permeability induced by vascular endothelial growth factor.

Spring K, Lapointe L, Caron C, Langlois S, Royal I
Cellular signalling 2014 Jun;26(6):1283-93

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Systematic validation of specific phenotypic markers for in vitro polarized human macrophages.

Ambarus CA, Krausz S, van Eijk M, Hamann J, Radstake TR, Reedquist KA, Tak PP, Baeten DL
Journal of immunological methods 2012 Jan 31;375(1-2):196-206

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Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.

Cabezón R, Sintes J, Llinàs L, Benitez-Ribas D
Immunology letters 2011 Jan 30;134(2):167-73

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human DEP-1/CD148 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human DEP-1/CD148 Monoclonal Antibody (Catalog # MAB1934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEP-1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.