HPA031613

antibody from Atlas Antibodies

Targeting: CCR4 CC-CKR-4, CD194, ChemR13, CKR4, CMKBR4, k5-5

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA031613

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA031613, RRID:AB_10601096

Product name

Anti-CCR4

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LQIYSADTPSSSYTQSTMDHDLHDAL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human tonsil and skeletal muscle tissues using HPA031613 antibody. Corresponding CCR4 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows moderate to strong cytoplasmic positivity in a subset of lymphoid cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in striated muscle fibers, as expected.

Sample type

HUMAN