PA5-32698

antibody from Invitrogen Antibodies

Targeting: CCR4 CC-CKR-4, CD194, ChemR13, CKR4, CMKBR4, k5-5

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-32698

Provider

Invitrogen Antibodies

Product name

CCR4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Ferret, Elephant, Panda (94%); Dog, Bovine, Bat, Hamster (89%); Mouse, Rat (83%). For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Prognostic value of immunoexpression of CCR4, CCR5, CCR7 and CXCR4 in squamous cell carcinoma of tongue and floor of the mouth.

Domingueti CB, Janini JB, Paranaíba LM, Lozano-Burgos C, Olivero P, González-Arriagada WA
Medicina oral, patologia oral y cirugia bucal 2019 May 1;24(3):e354-e363

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil using a CCR4 polyclonal antibody (Product # PA5-32698). Heat-induced antigen retrieval was performed prior to staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Low/negative, moderate and high staining intensity for CCR4 (A,B,C), CCR5 (D,E,F), CCR7 (G,H,I) and CXCR4 (J,K,L) (20x objective).