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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 10574-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUV39H1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GCSVCCKSS WNQLQDLCRL AKLSCPALGI SKRNLYDFEV EYLCDYKKIR EQEYYLVKWR GYPDSESTWE PRQNLKCVRI LKQFHKDLER ELLRRHHRSK TPRHLDPSLA NYLVQKAKQR RALRRWEQEL NAKRSHLGRI TVENEVDLDG PPRAFVYINE YRVGEGITLN QVAVGCECQD CLWAPTGGCC PGASLHKFAY NDQGQVRLRA GLPIYECNSR CRCGYDCPNR VVQKGIRYDL CIFRTDDGRG WGVRTLEKIR KNSFVM (7-271 aa encoded by B C006238)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.26 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C6 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.20 µg SUV39H1 antibody (Product # 10574-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SUV39H1 (IP:10574-1-AP, 4ug; Detection:10574-1-AP 1:500) with HeLa cells lysate 2800ug.
