16809-1-AP

antibody from Invitrogen Antibodies

Targeting: MAGED4 KIAA1859, MAGE-E1, MAGE1, MGC3210

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16809-1-AP

Provider

Invitrogen Antibodies

Product name

MAGED4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAEGSFSVQ SESYSVEDMD EGSDEVGEEE MVEGNDYEEF GAFGGYGTLT SFDIHILRAF GSLGPGLRIL SNEPWELENP VLAQTLVEAL QLDPETLANE TAARAANVAR AAASNRAARA AAAAARTAFS QVVASHRVAT PQVSGEDTQP TTYAAEAQGP TPEPPLASPQ TSQMLVTSKM AAPEAPATSA QSQTGSPAQE AATEGPSSAC AFSQAPCARE VDANRPSTAF LGQNDVFDFT QPAGVSGMAF PRPKRPAPAQ EAATEGPSAA SGVPQTGPGR EVAATRPKTT KSGKALAKTR WVEPQNVVAA AAAKAKMATS IPEPEGAAAA TAQHSAEPWA RMGGKRTKKS K (1-350 aa encoded by BC081566)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 16809-1-AP ( MAGED4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 16809-1-AP ( MAGED4 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using (Product # 16809-1-AP) (MAGED4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).