AP2114c

antibody from Abgent

Targeting: UBE2K HIP2, HYPG, UBC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2114c

Provider

Abgent

Proper citation

Abgent Cat#AP2114c, RRID:AB_352949

Product name

HIP2 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"HIP2 Antibody (D124) (Cat. #AP2114c) western blot analysis in 293,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the HIP2 antibody detected the HIP2 protein (arrow)."

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent image of Hela cell stained with HIP2 Antibody (Center)(Cat#AP2114c/SH030911H).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). HIP2 immunoreactivity is localized to Cytoplasm significantly."

Primary Ab dilution

1:10~50