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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6301 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6301, RRID:AB_1579999
- Product name
- UBE2K polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of UBE2K.
- Antigen sequence
C-SWDVETATELLLSN- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.
Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR
The Journal of biological chemistry 1996 Aug 9;271(32):19385-94
The Journal of biological chemistry 1996 Aug 9;271(32):19385-94
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- UBE2K polyclonal antibody (Cat # PAB6301) staining (1.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- UBE2K polyclonal antibody (Cat # PAB6301)(3.8 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
