NB300-840

antibody from Novus Biologicals

Targeting: UBE2K HIP2, HYPG, UBC1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-840

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-840, RRID:AB_2210773

Product name

Goat Polyclonal UBE2K/E2-25K Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.

Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR
The Journal of biological chemistry 1996 Aug 9;271(32):19385-94

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Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.

Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR
The Journal of biological chemistry 1996 Aug 9;271(32):19385-94

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UBE2K/E2-25K Antibody [NB300-840] - (1.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UBE2K/E2-25K Antibody [NB300-840] - (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6,