Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB500-487 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-487, RRID:AB_2236717
- Product name
- Mouse Monoclonal PAG1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp/ PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Antigen sequence
Located in the C-terminal domain.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A TRIF-independent branch of TLR3 signaling.
Yamashita M, Chattopadhyay S, Fensterl V, Zhang Y, Sen GC
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2825-33
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2825-33
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PAG1 Antibody (PAG-C1) [NB500-487] - Immunoprecipitation of human Cbp/PAG from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human Cbp/PAG (MEM-255). Note: Csk-binding protein (Cbp/PAG) is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-Cbp/PAG (PAG-C1) Lane 3: immunoprecipitation with anti-Cbp/PAG (polyclonal antibody).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAG1 Antibody (PAG-C1) [NB500-487] - staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.
