Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 606401 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#606401, RRID:AB_2229802
- Product name
- Purified anti-CBP
- Antibody type
- Polyclonal
- Antigen
- Recombinant (partial) , N-terminal
- Description
- CBP (also known as CREB-binding protein and CBP/p300) is a widely expressed nuclear protein that contains a bromodomain, a ZZ-type zinc domain, and TAZ-type zinc fingers. This 265 kD acetyltransferase enzyme, acetylates histones and non-histone proteins like NCOA3 coactivator and mediates c-AMP gene regulating by binding to phosphorylated CREB. CBP and phosphorylated CREB activates transcription of c-AMP responsive genes; defects in CBP result in Rubinstein-Taybi syndrome (mental retardation, craniofacial defects, heart defects). CBP is activated by TCR signaling and is involved in TNF-α gene expression and plays a central role in regulating gene responses to hypoxia. This protein is acetylated at multiple sites. CBP has been shown to interact with NCOA6, androgen receptor, STAT6, SRC1, Csk, FKHR, and c-jun and to form a complex with NCOA2, NCOA3, IKKA, IKKB, and IKBKG. In addition, CBP probably forms a complex with HIF1A and EP300. The Poly6064 antibody has been shown to be useful for Western blotting of the human and mouse CBP protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 50 µl
- Storage
- Upon receipt, store frozen at -20° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- MCF-7 cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against CBP. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated