Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00054820-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00054820-M01, RRID:AB_425994
- Product name
- NDE1 monoclonal antibody (M01), clone 2G11-1C11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NDE1.
- Antigen sequence
MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEE
LREFQEGSREYEAELETQLQQIETRNRDLLSENNR
LRMELETIKEKFEVQHSEGYRQISALEDDLAQTKA
IKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQ
RLNQAIERNAFLESELDEKENLLESVQRLKDEARD
LRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATG
SVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPL
TPAARISALNIVGDLLRKVGALESKLASCRNLVYD
QSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDK
GLDTSCRWLSKSTTRSSSSC- Isotype
- IgG
- Antibody clone number
- 2G11-1C11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references NDE1 and NDEL1: multimerisation, alternate splicing and DISC1 interaction.
Bradshaw NJ, Christie S, Soares DC, Carlyle BC, Porteous DJ, Millar JK
Neuroscience letters 2009 Jan 16;449(3):228-33
Neuroscience letters 2009 Jan 16;449(3):228-33
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NDE1 monoclonal antibody (M01), clone 2G11-1C11 Western Blot analysis of NDE1 expression in Hela ( Cat # L013V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NDE1 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NDE1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol