PA5-47665
antibody from Invitrogen Antibodies
Targeting: CLEC10A
CD301, CLECSF13, CLECSF14, HML, HML2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-47665 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD301 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody detects human CLEC10A/CD301 in direct ELISAs and Western blots and mouse CLEC10A in Western blots. Less than 1% cross-reactivity with recombinant human (rh) CLEC1, rhCLEC2, and rhCLEC3B is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human immature dendritic cells and human mature dendritic cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-human/mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47665) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for CLEC10A/CD301 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human immature dendritic cells and human mature dendritic cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-human/mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47665) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for CLEC10A/CD301 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of human immature dendritic cells were stained with Goat Anti-human/mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47665, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.