Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34615 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cullin 4A Recombinant Rabbit Monoclonal Antibody (JU07-33)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Hela, A431, HUVEC, human liver cance tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human cervix tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JU07-33
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
Submitted references Inhibition of cytoplasmic EZH2 induces antitumor activity through stabilization of the DLC1 tumor suppressor protein.
Tripathi BK, Anderman MF, Bhargava D, Boccuzzi L, Qian X, Wang D, Durkin ME, Papageorge AG, de Miguel FJ, Politi K, Walters KJ, Doroshow JH, Lowy DR
Nature communications 2021 Dec 3;12(1):6941
Nature communications 2021 Dec 3;12(1):6941
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cullin 4a in HeLa cell lysates. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cullin 4a in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cullin 4a in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cullin 4a in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human liver cancer tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human kidney tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human colon cancer tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human cervix tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human breast cancer tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Cullin 4a in HeLa cells (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
