Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-2267 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2267, RRID:AB_2086551
- Product name
- Rabbit Polyclonal Cullin 4a Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Efficient parvovirus replication requires CRL4Cdt2-targeted depletion of p21 to prevent its inhibitory interaction with PCNA.
Cullin-4A·DNA damage-binding protein 1 E3 ligase complex targets tumor suppressor RASSF1A for degradation during mitosis.
Adeyemi RO, Fuller MS, Pintel DJ
PLoS pathogens 2014 Apr;10(4):e1004055
PLoS pathogens 2014 Apr;10(4):e1004055
Cullin-4A·DNA damage-binding protein 1 E3 ligase complex targets tumor suppressor RASSF1A for degradation during mitosis.
Jiang L, Rong R, Sheikh MS, Huang Y
The Journal of biological chemistry 2011 Mar 4;286(9):6971-8
The Journal of biological chemistry 2011 Mar 4;286(9):6971-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cullin 4a Antibody [NB100-2267] - Detection of Human and Mouse Cul4a by WB (h&m) and Immunoprecipitation (h). Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Cul4a antibody NB100-2267 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). Cul4a was also immunoprecipitated by rabbit anti-Cul4a antibody NB100-2266.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267] - FFPE section of human breast tumor. Affinity purified rabbit anti-Cul4a used at a dilution of 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cullin 4a Antibody [NB100-2267] - Section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Cul4a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB