PAB28467

antibody from Abnova Corporation

Targeting: RBM22 Cwc2, FLJ10290, fSAP47, ZC3H16

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28467

Provider

Abnova Corporation

Product name

RBM22 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RBM22

Antigen sequence

NTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYG
KECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKL
KNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDV
NKEYYTQNMEREISNSDGTRPVGMLGKATSTSDML
LKL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with RBM22 polyclonal antibody at 1:100-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with RBM22 polyclonal antibody (Cat # PAB28467) at 1-4 ug/ml shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with RBM22 polyclonal antibody (Cat # PAB28467) shows strong nuclear positivity in neuronal cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)