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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-54257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSUN7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SEVQEVENLL NSFKIKLAAA LARCRIKHDA LSIYHILPET VRKQELRAST LPLYAWINTC KISPEEVYNN LKRRGYNKVK SVLHIDDKVF AVDQHCYDVL IFPSHLKNDL INIDLFKDYK LIFQDKSRSL A
- Concentration
- 0.10 mg/mL
Submitted references Identification of an RNA-Binding-Protein-Based Prognostic Model for Ewing Sarcoma.
Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures.
Chen Y, Su H, Su Y, Zhang Y, Lin Y, Haglund F
Cancers 2021 Jul 25;13(15)
Cancers 2021 Jul 25;13(15)
Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures.
Begik O, Lucas MC, Liu H, Ramirez JM, Mattick JS, Novoa EM
Genome biology 2020 May 7;21(1):97
Genome biology 2020 May 7;21(1):97
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NSUN7 in human cell line U-2 OS shows positivity in vesicles. Samples were probed using a NSUN7 Polyclonal Antibody (Product # PA5-54257).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NSUN7 in human testis tissue shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells. Samples were probed using a NSUN7 Polyclonal Antibody (Product # PA5-54257).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Analysis of RMP gene expression during spermatogenesis. a Schematic representation of the four main phases of spermatogenesis: (i) mitotic division of spermatogonia (SPG) into primary spermatocytes (PSC), (ii) meiotic division of PSCs into secondary spermatocytes (SC), (iii) meiotic division SCs into round spermatids (RST), and (iv) spermiogenesis, in which round spermatids (RST) mature into elongated spermatids (EST). b Heatmap of RMP expression levels in mouse testis. RMPs were clustered into 4 groups based on k-means analysis of their normalized average mRNA expression values. c Violin plots of the expression patterns of each of the 4 identified clusters. d RNA median expression barplot and immunohistochemistry of NSUN7, NSUN2, and METTL14, depicting distinct protein expression levels along the different sections of the testis and epididymis, as well as different subcellular localizations. Brown color indicates a specific staining of the antibody whereas blue represents hematoxylin counterstain
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Examples of histology imaging and follow-up in patients with ES. ( A ). Comparison of the survival status and the NSUN7 IHC scores in 24 ES patients (Fisher's exact test). ( B ). Kaplan-Meier curve shows the overall survival of NSUN7 positive and negative in the subgroup of IHC staining. ( C ). Comparison between NSUN7 immunoreactivity in negative, weak, and moderate scoring at 100x and 400x magnification.
