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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NBP1-87170 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87170, RRID:AB_11008062
- Product name
- Rabbit Polyclonal RABL4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human RABL4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Taulet N, Vitre B, Anguille C, Douanier A, Rocancourt M, Taschner M, Lorentzen E, Echard A, Delaval B
Nature communications 2017 Dec 4;8(1):1928
Nature communications 2017 Dec 4;8(1):1928
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RABL4 Antibody [NBP1-87170] - Analysis in human cell lines HEK293 and Caco-2 using anti-IFT27 antibody. Corresponding IFT27 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RABL4 Antibody [NBP1-87170] - Analysis in control (vector only transfected HEK293T lysate) and IFT27 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RABL4 Antibody [NBP1-87170] - IFT proteins associate with central spindle MTs in anaphase and are required for their organization. Western blots (left) showing the amount of IFT27 and IFT88 in LLC-PK1 cells transfected with control (CT), IFT27 or IFT88 siRNA. alpha-tubulin: loading control. Immunofluorescence images (right) showing a decrease of IFT88 staining in an anaphase cell upon IFT88 depletion. Scale bars: 10?um for main images, 3?um for insets. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-017-01479-3), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RABL4 Antibody [NBP1-87170] - IFT proteins are required for the timely relocalization of the kinase Aurora B to the central spindle. Myc immunoprecipitation performed on mitotic HeLa Kyoto cells transfected with Myc or with Myc-Aurora B. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-017-01479-3), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RABL4 Antibody [NBP1-87170] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
