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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- TA502764 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502764, RRID:AB_11126707
- Product name
- SH2B3 (LNK) mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)
- Antibody type
- Monoclonal
- Description
- SH2B3 (LNK) mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SH2B3
- Isotype
- IgG
- Antibody clone number
- OTI2D8
- Vial size
- 100 µl
- Concentration
- 0.37 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SH2B3 mouse monoclonal antibody (TA502764) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3(RC218359 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SH2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502764)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SH2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502764)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-SH2B3 antibody(TA502764),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA502764), and then analyzed by flow cytometry.
- Validation comment
- FC
