PA5-78273

antibody from Invitrogen Antibodies

Targeting: WASF1 KIAA0269, SCAR1, WAVE, WAVE1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78273

Provider

Invitrogen Antibodies

Product name

WAVE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: mouse brain, rat brain

Concentration

1.06 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WAVE1 in various tissue extracts using 50 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with WAVE1 polyclonal antibody (Product # PA5-78273) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using WAVE1 Polyclonal Antibody (Product # PA5-78273). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of WAVE1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WAVE1 Polyclonal Antibody detects WAVE1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WAVE1 protein stained by WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WAVE1 Polyclonal Antibody detects WAVE1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WAVE1 Polyclonal Antibody detects WAVE1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.