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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- A302-268A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MLLT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MLLT1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLLT1 antibody (Product # A302-268A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MLLT1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLLT1 antibody A302-268A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. MLLT1 was also immunoprecipitated by rabbit anti-MLLT1 antibody A302-267A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MLLT1 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-MLLT1 (Product # A302-268A; lot 2) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MLLT1 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MLLT1 (Cat. No. A302-268A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MLLT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLLT1 antibody (Product # A302-268A lot 2) used for IP at 6 µg per reaction. MLLT1 was also immunoprecipitated by a previous lot of this antibody (A302-268A lot 1) and a second antibody against a different epitope of MLLT1 (Product # A302-267A). For blotting immunoprecipitated MLLT1 (Product # A302-268A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
