Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- PA5-34559 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GABARAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is K562 cell lysate. PA5-34559 can be used with blocking peptide PEP-1602.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of K562 cell lysate using a GABARAP polyclonal antibody (Product # PA5-34559) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GABARAP in HeLa cell lysate with GABARAP Polyclonal Antibody (Product # PA5-34559) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GABARAP Rabbit Polyclonal Antibody (Product # PA5-34559) and 14 kDa band corresponding to GABARAP were observed in HeLa cell line tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and HeLa treated with 50 µM Chloroquine for 20 hours (Lane 2) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/ml) and detected by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Uncharacterized bands (*) at 40 kDa and 58 kDa were observed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of GABARAP in human brain tissue with GABARAP Polyclonal Antibody (Product # PA5-34559) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of GABARAP in mouse brain tissue with GABARAP Polyclonal Antibody (Product # PA5-34559) at 20 µg/mL. Green: GABARAP Red: Phylloidin staining Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of GABARAP in mouse brain tissue with GABARAP Polyclonal Antibody (Product # PA5-34559) at 5 µg/mL.