PAB0730

antibody from Abnova Corporation

Targeting: GABARAP ATG8A, MM46

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0730

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0730, RRID:AB_1112893

Product name

GABARAP polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of GABARAP.

Storage

Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot under reducing conditions on 293 cell lysate using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 100.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 100.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Confocal microscopy on immunofluorescently detected GABARAP in cytospin-isolated human white blood cells using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 200, incubated for 1 hr at room temperature. The cells were double - stained for GABARAP appearing in red (A) and MHC class II appearing in green (B) . The cells were counter stained with Hoechst Dye (C, blue colour) . Here, the merged picture is presented.

Validation comment

Immunofluorescence