Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB0730 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0730, RRID:AB_1112893
- Product name
- GABARAP polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GABARAP.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot under reducing conditions on 293 cell lysate using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 100.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 100.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal microscopy on immunofluorescently detected GABARAP in cytospin-isolated human white blood cells using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 200, incubated for 1 hr at room temperature. The cells were double - stained for GABARAP appearing in red (A) and MHC class II appearing in green (B) . The cells were counter stained with Hoechst Dye (C, blue colour) . Here, the merged picture is presented.
- Validation comment
- Immunofluorescence