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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- AP32182PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti GABARAP (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 1~31 amino acids from the N-terminal region of Human GABARAP
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N at 1/75 dilution in 5% scimmed milk powder. Secondary antibody Goat anti-Rabbit-HRP ECL detection 30 sec on X-ray film.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot done using standard conditions on WT MEFs, starved with EBSS for 2 hours with and without leupeptin before lysing. Primary antibodies used were GABARAP Antibody (N-term) Cat.-No AP32182PU-N, 1/250 in 5% milk (in PBS + 0.1% Tween-20) and  Rabbit anti-Actin. Secondary detection by anti-Rabbit-HRP, ECL exposure 2 minutes (provided by Fiona McAlpine & Sharon Tooze).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of GABARAP (arrow) using Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N. 293 cell lysates (2 µg/Lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- GABARAP Antibody was tested in Mouse MEFs and Mouse motor neurons. Cells were fixed in 3% PFA and permeabilized with 100% methanol. Antibodies used were GABARAP Antibody (N-term) Cat.-No AP32182PU-N (1/500, labeled with anti-Rabbit 488 Alexa) and Covance anti-Tuj1 (1/1000, labeled with anti-Mouse 647).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N on paraformaldehyde-fixed, saponin-permeabilized HeLa cells. Comparison of control (full-medium) and Autophagy induced (EBSS 1 h) cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP Antibody (N-term) Cat.-No AP32182PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
