TA503373

antibody from OriGene

Targeting: GTF2F1 BTF4, RAP74, TF2F1, TFIIF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503373

Provider

OriGene

Proper citation

OriGene Cat#TA503373, RRID:AB_11128162

Product name

GTF2F1 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)

Antibody type

Monoclonal

Description

GTF2F1 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)

Host

Mouse

Conjugate

Unconjugated

Epitope

GTF2F1

Isotype

IgG

Antibody clone number

OTI1C5

Vial size

100 µl

Concentration

0.78 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GTF2F1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (RC201294, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-GTF2F1 mouse monoclonal antibody (TA503373) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1(RC201294).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC201294 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GTF2F1 antibody(TA503373), and then analyzed by flow cytometry.

Validation comment

FC