LS-C797468

antibody from LSBio

Targeting: GTF2F1 BTF4, RAP74, TF2F1, TFIIF

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C797468

Provider

LSBio

Product name

RAP74 / GTF2F1 Antibody (clone OTI2H3, Carrier-free) LS-C797468

Antibody type

Monoclonal

Description

Purified from ascites.

Reactivity

Human, Mouse, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2H3

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of extracts (35 ug) from 9 different cell lines by using anti-GTF2F1 monoclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of extracts (35 ug) from 9 different cell lines by using anti-GTF2F1 monoclonal antibody.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1.