A302-756A

antibody from Invitrogen Antibodies

Targeting: GTF2F1 BTF4, RAP74, TF2F1, TFIIF

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-756A

Provider

Invitrogen Antibodies

Product name

RAP74 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RAP74 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RAP74 (Cat. No. A302-756A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RAP74 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAP74 antibody A302-756A used for IP at 3 µg/mg lysate. RAP74 was also immunoprecipitated by rabbit anti-RAP74 antibody A302-757A, which recognizes a downstream epitope. For blotting immunoprecipitated RAP74, A302-757A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.